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Tag Archives: somatic cell

Analysis of allelic expression patterns in clonal somatic cells by single-cell RNA–seq

September 28, 2016 Leave a comment 4,221 Views

rna-seq

Cellular heterogeneity can emerge from the expression of only one parental allele. However, it has remained controversial whether, or to what degree, random monoallelic expression of autosomal genes (aRME) is mitotically inherited (clonal) or stochastic (dynamic) in somatic cells, particularly ...

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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  • rna-seq

    Novel guidelines help select optimal deconvolution method

    1 day ago
  • rna-seq

    An RNA-Seq framework for profiling target selectivity and physiological effects of antisense peptide nucleic acids (PNAs)

    1 day ago
  • rna-seq

    Single cell sequencing unravels molecular identity of rare renal cell types

    2 days ago
  • rna-seq

    SMIXnorm – fast and accurate RNA-Seq data normalization for FFPE Samples

    2 days ago
  • rna-seq

    TEA-seq – simultaneous trimodal single-cell measurement of transcripts, epitopes, and chromatin accessibility

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    First use of both bulk and single-cell RNA sequencing of human bladder tumors to study resistance to immunotherapy

    3 days ago
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    Post-doc position available – transcriptome bioinformatics and RNA sequencing

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