Tag Archives: spike-in controls

Spike-in controls are synthetic nucleic-acid sequences that are added to a user’s sample and constitute internal standards for subsequent steps in the next generation sequencing workflow. The Anaquin software toolkit can be used to analyze the performance of spike-in controls ...

Single-cell gene expression studies promise to reveal rare cell types and cryptic states, but the high variability of single-cell RNA-seq measurements frustrates efforts to assay transcriptional differences between cells. University of Washington researchers have developed the Census algorithm to convert ...

DATE: October 19, 2016 TIME:  9:00 AM PT, 12:00 PM ET, 6:00 PM CEST PRESENTED BY:Lukas Paul, PhD Head of Services, Lexogen Hardly anyone would run an RNA gel without a ladder, but transcriptomes are mostly sequenced without the use ...

RNA sequencing (RNA-seq) can be used to assemble spliced isoforms, quantify expressed genes and provide a global profile of the transcriptome. However, the size and diversity of the transcriptome, the wide dynamic range in gene expression and inherent technical biases ...

Highly multiplexed assays for quantitation of RNA transcripts are being used in many areas of biology and medicine. Using data generated by these transcriptomic assays requires measurement assurance with appropriate controls. Methods to prototype and evaluate multiple RNA controls were ...

Genome-scale “-omics” measurements are challenging to benchmark due to the enormous variety of unique biological molecules involved. Mixtures of previously-characterized samples can be used to benchmark repeatability and reproducibility using component proportions as truth for the measurement. Researchers at the ...

RNA is complex, and its sequencing is even more complex. One of the biggest complications originates from the fact that RNA consists of numerous variations such as alternative splicing and isoforms which are introduced during the process of transcription and ...

Genome-scale ?-omics? measurements are challenging to benchmark due to the enormous variety of unique biological molecules involved. Mixtures of previously-characterized samples can be used to benchmark repeatability and reproducibility using component proportions as truth for the measurement. Scientists at the ...

A new, innovative “dashboard” from the National Institute of Standards and Technology (NIST) won’t help you drive your car, but it will help enable reproducible research in biology. Artist’s conception of an RNA polymerase (globular molecule) creating strands of RNA ...