Tag Archives: splicing

High-throughput sequencing of transcriptomes (RNA-Seq) has become a powerful tool to study gene expression. Here researchers from the University of Michigan present an R package, rSeqNP, which implements a non-parametric approach to test for differential expression and splicing from RNA-Seq ...

Analysis of RNA sequencing (RNA-Seq) data revealed that the vast majority of human genes express multiple mRNA isoforms, produced by alternative pre-mRNA splicing and other mechanisms, and that most alternative isoforms vary in expression between human tissues (Pan et al. ...

RNA-sequencing data analysis method BitSeq developed by Academy Research Fellow Antti Honkela?s research group and University of Manchester researchers has been found to be the most accurate gene transcript expression estimation method in a large international assessment. The method is ...

Researchers at the Academy of Sciences of the Czech Republic propose ways to improve read mapping accuracy and sensitivity such as modifying a reference genome at RNA editing sites, using splicing- and ambiguity-competent aligners, and masking chloroplast- or nucleus-derived sequences. ...

RNA-Seq is proving to be a valuable tool for the investigation of known and novel transcripts affecting cell development, disease status or disease mechanisms. Being able to address a range of questions, from differential expression to differential splicing, offers researchers ...

The development of novel high-throughput sequencing (HTS) methods for RNA (RNA-Seq) has provided a very powerful mean to study splicing under multiple conditions at unprecedented depth. However, the complexity of the information to be analyzed has turned this into a ...