A key component in many RNA-Seq based studies is contrasting multiple replicates from different experimental conditions. In this setup, replicates play a key role as they allow to capture underlying biological variability inherent to the compared conditions, as well as ...Read More »
RNA-Sequencing (RNA-Seq) has become a routine technology for investigating gene expression differences in comparative transcriptomic studies. Differential expression (DE) analysis of the isoforms of genes is just emerging now that expression (read counts) can be estimated with higher accuracy at ...Read More »
How many biological replicates are needed in an RNA-seq experiment and which differential expression tool should you use?
RNA-seq is now the technology of choice for genome-wide differential gene expression experiments, but it is not clear how many biological replicates are needed to ensure valid biological interpretation of the results or which statistical tools are best for analyzing ...Read More »
(RNA-seq) has become a routine technique in transcriptome analysis, where identifying differential expression (DE) remains a major task. As expression is a stochastic process in nature, the technological improvement in RNA-seq cannot bypass the presence of biological variability. It has ...Read More »
Studies of expression quantitative trait loci (eQTLs) offer insight into the molecular mechanisms of loci that were found to be associated with complex diseases and the mechanisms can be classified into cis- and trans-acting regulation. At present, high-throughput RNA sequencing ...Read More »