RNA-Seq data analysis has become vital to understanding gene structure and expression patterns in transcriptomic studies. A typical reference genome guided RNA-Seq workflow...
Read More »Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown
High-throughput sequencing of mRNA (RNA-seq) has become the standard method for measuring and comparing the levels of gene expression in a wide variety of species and conditions. RNA-seq experiments generate very large, complex data sets that demand fast, accurate and ...
Read More »StringTie – improved reconstruction of a transcriptome from RNA-Seq reads
Methods used to sequence the transcriptome often produce more than 200 million short sequences. Researchers at Johns Hopkins University have developed StringTie, a computational method that applies a network flow algorithm originally developed in optimization theory, together with optional de ...
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