Researchers from the Scripps Research Institute describe a method for making Illumina-compatible sequencing libraries from RNA. This protocol can be used for standard RNAseq analysis for detecting differentially expressed genes. In addition, this protocol is ideally suited for adapting to ...
Read More »Transcriptome Sequencing Links Bipolar Disorder to Unexpected Brain Region
While bipolar disorder is one of the most-studied neurological disorders—the Greeks noticed symptoms of the disease as early as the first century—it’s possible that scientists have overlooked an important part of the brain for its source. Scientists from the Florida ...
Read More »OncoRep – an n-of-1 reporting tool to support genome-guided treatment for breast cancer patients using RNA-sequencing
Breast cancer comprises multiple tumor entities associated with different biological features and clinical behaviors, making individualized medicine a powerful tool to bring the right drug to the right patient. Next generation sequencing of RNA (RNA-Seq) is a suitable method to ...
Read More »Omics Pipe: A Community-based Framework for Reproducible Multi-Omics Data Analysis
Omics Pipe is a computational framework that automates multi-omics data analysis pipelines on high performance compute clusters and in the cloud. It supports best practice published pipelines for RNA-seq, miRNA-seq, Exome-seq, Whole Genome sequencing, ChIP-seq analyses and automatic processing of ...
Read More »OncoRep: An n-of-1 reporting tool to support genome-guided treatment for breast cancer patients using RNA-sequencing
Breast cancer comprises multiple tumor entities associated with different biological features and clinical behaviors, making individualized medicine a powerful tool to bring the right drug to the right patient. Next generation sequencing of RNA (RNA-Seq) is a suitable method to ...
Read More »Omics Pipe – A Computational Framework for Reproducible Multi-Omics Data Analysis
Omics Pipe is a computational framework for reproducible multi-omics data analysis that supports several next generation sequencing best practice pipelines, such as RNA-seq, miRNA-seq, Exome-seq, whole genome-seq and ChIP-seq. The purpose of Omics Pipe is to provide researchers with an ...
Read More »Library construction for next-generation sequencing: Overviews and challenges
High-throughput sequencing, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily improved, with costs dropping and the number and range of sequencing applications increasing exponentially. Here, researchers from The Scripps Research Institute ...
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