Tag Archives: Transcription

Power analysis at the isoform level

rna-seq

RNA-Sequencing (RNA-Seq) has become a routine technology for investigating gene expression differences in comparative transcriptomic studies. Differential expression (DE) analysis of the isoforms of genes is just emerging now that expression (read counts) can be estimated with higher accuracy at ...

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Fast Read Stitcher – an annotation agnostic algorithm for detecting nascent RNA transcripts in global nuclear run-on sequencing (GRO-seq)

rna-seq

Researchers at the University of Colorado, Boulder have developed a fast and simple algorithm to detect nascent RNA transcription in global nuclear run-on sequencing (GRO-seq). GRO-seq is a relatively new protocol that captures nascent transcripts from actively engaged polymerase, providing a ...

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mNET-Seq – snapshots of transcription

rna-seq

Transcription is a highly dynamic process. Consequently, researchers at the University of Oxford have developed native elongating transcript sequencing technology for mammalian chromatin (mNET-seq), which generates single-nucleotide resolution, nascent transcription profiles. Nascent RNA was detected in the active site of ...

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