Tag Archives: transcriptome assembly

DNASTAR announced the release of Lasergene 13 today, which significantly advances scientists’ ability to perform RNA research on model and non-model organisms on standard desktop computers. Significant software enhancements include the ability to perform de novo transcriptome assembly of RNA ...

High-throughput RNA-seq technology has provided an unprecedented opportunity to reveal the very complex structures of transcriptomes. However, it is an important and highly challenging task to assemble vast amounts of short RNA-seq reads into transcriptomes with alternative splicing isoforms. Now, ...

The recent advances in high throughput RNA sequencing (RNA-Seq) have generated huge amounts of data in a very short span of time for a single sample. These data have required the parallel advancement of computing tools to organize and interpret ...

SOLiD DNA sequences are typically analyzed using a reference genome, while they are not recommended for de novo assembly of genomes or transcriptomes. This is mainly due to the difficulty in translating the SOLiD color-space data into normal base-space sequences. ...

Next generation sequencing methods, such as RNA-seq, have permitted the exploration of gene expression in a range of organisms which have been studied in ecological contexts but lack a sequenced genome. However, the efficacy and accuracy of RNA-seq annotation methods ...

Owing greatly to the advancement of next-generation sequencing (NGS), the amount of NGS data is increasing rapidly. Although there are many NGS applications, one of the most commonly used techniques ‘RNA sequencing (RNA-seq)’ is rapidly replacing microarray-based techniques in laboratories ...

Three principal approaches have been proposed for inferring the set of transcripts expressed in RNA samples using RNA-seq. The simplest approach uses curated annotations, which assumes the transcripts in a sample are a subset of the transcripts listed in a ...

Methods used to sequence the transcriptome often produce more than 200 million short sequences. Researchers at Johns Hopkins University have developed StringTie, a computational method that applies a network flow algorithm originally developed in optimization theory, together with optional de ...

RNA-Seq based transcriptome assembly has become a fundamental technique for studying expressed mRNAs (i.e ., transcripts or isoforms) in a cell using high-throughput sequencing technologies, and is serving as a basis to analyze the structural and quantitative differences of expressed ...

RNA Bioinformatics Edited by Ernesto Picardi ISBN: 978-1-4939-2290-1 (Print) 978-1-4939-2291-8 (Online) Analysis of High-Throughput RNA Sequencing Data Quality Control of RNA-Seq Experiments Xing Li, Asha Nair, Shengqin Wang, Liguo Wang Pages 137-146 Accurate Mapping of RNA-Seq Data Kin Fai Au ...