Tag Archives: transcriptome profiling

Takara Bio is pleased to announce the launch of a high-throughput, whole transcriptome profiling RNA-seq kit. The kit generates full-length cDNA libraries using FACS-sorted single cells or ultra-low input RNA. In our webinar, Dr. Ankita Das (Takara Bio USA, Inc.) ...

Most transcriptome studies involve sequencing and quantification of steady-state mRNA by isolating and sequencing poly (A) RNA. Although this type of sequencing data is informative to determine steady-state mRNA levels it does not provide information on transcriptional output and thus ...

RNA-sequencing is a revolutionary tool to follow differential expression after treatment with cancer chemopreventive agents. It allows a real genome-wide screening independent of prior assumptions and is well suited for analyzing coding but also long noncoding RNAs. It still consents ...

Next-generation RNA-sequencing (RNA-seq) has revolutionized transcriptome profiling, gene expression analysis, and RNA-based diagnostics. Here, researchers at the University of Texas have developed a new RNA-seq method that exploits thermostable group II intron reverse transcriptases (TGIRTs) and used it to profile ...

While RNA-sequencing (RNA-seq) is becoming a powerful technology in transcriptome profiling, one significant shortcoming of the first-generation RNA-seq protocol is that it does not retain the strand specificity of origin for each transcript. Without strand information it is difficult and ...

In recent years, different technologies have been used to measure genome-wide gene expression levels and to study the transcriptome across many types of tissues and in response to in vitro treatments. However, a full understanding of gene regulation in any ...

Since the appearance of the ENCODE project and due to follow-up work, a shift from the pervasive transcription observed from RNA-Seq data to its functional validation is gradually occurring. However, much less attention has been turned to the problem of ...

Guidelines for determining sequencing depth facilitate transcriptome profiling of single cells in heterogeneous populations. In recent years, single-cell RNA-seq has emerged as a powerful, new approach for characterizing the cell types present in a mixed population. These studies usually involve ...

from BitsizeBio by Kristin Harper You’re about to start that big project you’ve been dreaming of for years. You’ve identified a potential miracle compound and want to figure out how it affects gene expression. But how are you going to ...

from Genetic Engineering News RNA sequencing (RNA-seq), a means of depicting the transcriptome, is being used with increasing frequency to characterize a growing array of conditions—everything from prenatal birth defects to disorders of the elderly. Yet the technique, which is ...