Trap-Seq – Translating Ribosome Affinity Purification

April 6, 2015 Leave a comment 6,488 Views

Researchers from the University of Michigan isolated mRNA from mouse hypothalamic and brainstem LepRb cells by Translating Ribosome Affinity Purification (TRAP) and analyzed it by RNA-seq (TRAP-seq). Leptin acts via its receptor (LepRb) on multiple subpopulations of LepRb neurons in ...

TRAP-SEQ – Translating Ribosome Affinity Purification (TRAP) Followed by RNA Sequencing Technology

March 17, 2015 Leave a comment 12,597 Views

Translating Ribosome Affinity Purification (TRAP) is a technology to isolate the population of mRNAs associated with at least one 80S ribosome, referred as the translatome. TRAP is based on the expression of an epitope-tagged version of a ribosomal protein and ...

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Tag Archives: TRAP-SEQ

Trap-Seq – Translating Ribosome Affinity Purification

TRAP-SEQ – Translating Ribosome Affinity Purification (TRAP) Followed by RNA Sequencing Technology

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The modulatory role of ions in stem cell transcriptome dynamics by regulating lineage-specific gene expression patterns

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