Small RNA-seq – The RNA 5′-end adapter ligation problem and how to circumvent it

May 16, 2019 Leave a comment 1,150 Views

The preparation of small RNA cDNA sequencing libraries depends on the unbiased ligation of adapters to the RNA ends. Small RNA with 5' recessed ends are poor substrates for enzymatic adapter ligation, but this 5' adapter ligation problem can...

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Small RNA-seq – The RNA 5′-end adapter ligation problem and how to circumvent it

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