Tag Archives: tutorial

Topic: RNA Sequencing Analysis Presented by: Tom Kono, Ph.D. Date: 11/6/18 Minnesota Supercomputing Institute 

The RNA-Seq analysis tutorial covers the basics of differential expression analysis and touches on other RNA-seq topics such as transcriptome assembly. Topics Covered: RNA-Seq vs Microarray technology Experimental Design Sequencing Technology Data Quality Assessments Mapping RNA-Seq data to a genome ...

RNA-seq analysis of human breast cancer data

RNA-seq analysis is becoming a standard method for global gene expression profiling. However, open and standard pipelines to perform RNA-seq analysis by non-experts remain challenging due to the large size of the raw data files and the hardware requirements for ...

A brief introduction to the Sleuth R Shiny app for doing exploratory data analysis of your RNA-Seq data. This tutorial assumes that the data have been already quantified with kallisto and processed into a sleuth object with the sleuth r ...

Check the full description for links to all the resources and the protocol etc. This tutorial follows the Delhomme et al. Epigenesys protocol for RNA sequencing data preprocessing in order to finally perform a differential expression study. To demonstrate, we ...

This is the material for two workshops given at MSU by Titus Brown and Matt Scholz. The first workshop (Dec 4/5) was on model organism mRNAseq, and the second workshop (Dec 10/11) will be on semi-model organism mRNAseq. Both workshops ...

A Brief History of Sequencing and Gene Expression. Tag Based Sequencing Approaches Serial Analysis of Gene Expression (SAGE) Cap Analysis of Gene Expression (CAGE) Massively parallel signature sequence (MPSS)

Canadian Bioinformatics Workshop Series Date June 19-20, 2014 – Vancouver, British Columbia Faculty Malachi Griffith and Obi Griffith Course Objectives High-throughput sequencing of RNA libraries (RNA-seq) has become increasingly common and largely supplanted gene microarrays for transcriptome profiling. When processed ...

RAP: RNA-Seq Analysis Pipeline RNA-Seq technology is becoming widely used in various transcriptomics studies; however, analyzing and interpreting the RNA-Seq data face serious challenges due to transcriptome complexity. A complete RNA-seq analysis involves several steps and the data can be ...