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Tag Archives: Ultralow input

CLEAR – a workflow for identifying robust/low-noise transcripts in limiting-cell RNA-seq data

February 14, 2020 Leave a comment 1,634 Views

rna-seq

Direct cDNA preamplification protocols developed for single-cell RNA-seq have enabled transcriptome profiling of precious clinical samples and rare cell populations without the need for sample pooling or RNA extraction. Ohio State University researchers...

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Recent RNA-SEQ Pubs

Network pharmacology and RNA-sequencing reveal the molecular mechanism of Xuebijing injection on COVID-19-induced cardiac dysfunction
4 March 2021
Direct Comparative Analyses of 10X Genomics Chromium and Smart-seq2
4 March 2021
Metabolic adjustments during starvation in Daphnia pulex
4 March 2021
SwarnSeq: An improved statistical approach for differential expression analysis of single-cell RNA-seq data
4 March 2021
The overexpression of DNA repair genes in invasive ductal and lobular breast carcinomas: Insights on individual variations and precision medicine
4 March 2021
Detecting cell-type-specific allelic expression imbalance by integrative analysis of bulk and single-cell RNA sequencing data
4 March 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

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    Learn more about available bioinformatics tools and pipelines to analyze RNA sequencing data

    8 hours ago
  • rna-seq

    New search engine for single cell atlases

    9 hours ago
  • rna-seq

    Yale researchers identify tumor reactive immune cells with RNA sequencing to help fight against advanced melanoma

    10 hours ago
  • rna-seq

    New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens

    1 day ago
  • rna-seq

    RNA sequencing brings hope for endangered wild cats

    1 day ago
  • rna-seq

    Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs

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    Uncovering the Anti-Myeloma Resistance Files

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