We are pursuing a modeling, data centric, approach that integrates human single cell RNA-Seq data together with in-vitro cellular models. In this unique set-up, hypothesis arising from patient samples will be tested on in-vitro models, thus providing a unique...
Read More »End Sequence Analysis ToolKit (ESAT) – a computational pipeline for single cell RNA-seq experiments
RNA-seq protocols that focus on transcript termini are well-suited for applications in which template quantity is limiting. Here researchers from UMass Medical School show that, when applied to end-sequencing data, analytical methods designed for global RNA-seq produce computational artifacts. To ...
Read More »piPipes – a set of pipelines for piRNA and transposon analysis via small RNA-seq, RNA-seq, degradome- and CAGE-seq, ChIP-seq and genomic DNA sequencing
PIWI-interacting RNAs (piRNAs), 23-36 nt small silencing RNAs, repress transposon expression in the metazoan germ line, thereby protecting the genome. Although high-throughput sequencing has made it possible to examine the genome and transcriptome at unprecedented resolution, extracting useful information from ...
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