Tag Archives: UMI

Single-cell RNA-seq (scRNA-seq) profiles gene expression of individual cells. Unique molecular identifiers (UMIs) remove duplicates in read counts resulting from polymerase chain reaction, a major source of noise. For scRNA-seq data lacking UMIs...

RNA-Seq technology is routinely used to characterize the transcriptome, and to detect gene expression differences among cell types, genotypes and conditions. Advances in short-read sequencing instruments such as Illumina Next-Seq have yielded easy-to-operate machines, with high throughput, at a lower ...

Canopy Biosciences™ LLC, a leading provider of gene editing and gene expression products and services, announced today that they had exclusively licensed technology for error correction in next generation...

By offering high sequencing speed and ultra-high-throughput at a low price, Illumina next-generation sequencing platforms have been widely adopted in recent years. However, an experiment with multiplexed library could be at risk of...

Recent single-cell RNA-seq protocols based on droplet microfluidics use massively multiplexed barcoding to enable simultaneous measurements of transcriptomes for thousands of individual cells. The increasing complexity of such data creates challenges for subsequent computational processing...

In single-cell RNA sequencing (scRNA-seq) studies, only a small fraction of the transcripts present in each cell are sequenced. This leads to unreliable...

St. Jude Children’s Research Hospital investigators have developed a software package to help identify biomarkers that differentiate between cell...

Single cell RNA sequencing approaches are instrumental in studies of cell-to-cell variability. 5′ selective transcriptome profiling approaches allow simultaneous definition of the transcription start size and have advantages over 3′ selective approaches which just provide internal sequences close to the ...

As part of the process of preparing scRNA-seq libraries, a diverse template is typically amplified by PCR. During amplification, spurious chimeric molecules can be formed between molecules originating in different cells. While several computational and experimental strategies have been suggested ...

The yield obtained from next generation sequencers has increased almost exponentially in recent years, making sample multiplexing common practice. While barcodes (known sequences of fixed length) primarily encode the sample identity of sequenced DNA fragments, barcodes made of random sequences ...