Tag Archives: University of California

RNA-Seq advances biofuel research

rna-seq

Research team taps EMSL, DOE JGI to harness promising fungi found in manure Nature’s figured it out already, how to best break down food into fuel. Now scientists have caught up, showing that fungi found in the guts of goats, ...

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NMFP – a non-negative matrix factorization based preselection method to identify mRNA isoforms from RNA-seq data

rna-seq

The advent of next-generation RNA sequencing (RNA-seq) has greatly advanced transcriptomic studies, including system-wide identification and quantification of mRNA isoforms under various biological conditions. A number of computational methods have been developed to systematically identify mRNA isoforms in a high-throughput ...

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BrAD-seq (Breath Adapter Directional sequencing) – a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction

rna-seq

Next Generation Sequencing (NGS) is driving rapid advancement in biological understanding and RNA-sequencing (RNA-seq) has become an indispensable tool for biology and medicine. There is a growing need for access to these technologies although preparation of NGS libraries remains a ...

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A method for extracting high-quality RNA from diverse plants for next-generation sequencing and gene expression analyses

To study expression in plants, high-quality RNA must be extracted in quantities sufficient for subsequent cDNA library construction. Field-based collections are often limited in quantity and quality of tissue and are typically preserved in RNAlater. Obtaining sufficient and high-quality yield ...

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