Polyploidy has played a pivotal and recurring role in angiosperm evolution. Allotetraploids arise from hybridization between species and possess duplicated gene copies (homeologs) that serve redundant roles...
Read More »RNA-Seq reveals that a common human med offers hope to cancer-stricken turtles
Tumours in sea turtles may be able to be treated using cancer treatments available to humans. A study published in the journal Communications...
Read More »An RNA-Seq Primer
Pharmacogenomics has revealed compelling genetic signals associated with variability in drug response. Gene expression studies represent an additional approach to identify candidate genes accounting for drug response variability. Researchers from the University of Florida discuss insights that might be gained through analysis of ...
Read More »Detecting Alternatively Spliced Transcript Isoforms from Single-Molecule Long-Read Sequences without a Reference Genome
Alternative splicing (AS) is a major source of transcript and proteome diversity, but examining AS in species without well-annotated reference genomes remains difficult. Research on both human and mouse has demonstrated the advantages of using Iso-Seq™ data for isoform-level transcriptome ...
Read More »UF seeks Research Assistant Scientist to conduct RNA-seq space flight experiments
Department: The Department of Materials Science and Engineering Job Number: 501708 Regular/Temporary: Regular Apply On or Before: March 28, 2017 Responsibilities: The Department of Materials Science and Engineering at the University of Florida is seeking a non-tenure track, full-time, 75% ...
Read More »Statistical method for reconstructing gene regulatory networks
In recent years, next generation sequencing (NGS) has gradually replaced microarray as the major platform in measuring gene expressions. Compared to microarray, NGS has many advantages, such as less noise and higher throughput. However, the discreteness of NGS data also ...
Read More »AAEP Foundation to Fund RNA-Seq Research on Lameness in Horses
Support-limb laminitis was the type of laminitis 2006 Kentucky Derby winner Barbaro (seen here at the University of Pennsylvania’s New Bolton Center) succumbed to while healing from a severe fracture in a hind limb. The American Association of Equine Practitioners ...
Read More »A survey of best practices for RNA-seq data analysis
RNA-sequencing (RNA-seq) has a wide variety of applications, but no single analysis pipeline can be used in all cases. A multi-national team led by researchers from the University of Florida reviews all of the major steps in RNA-seq data analysis, including ...
Read More »Application of an RNA amplification method for reliable single-cell transcriptome analysis
A team led by researchers at the University of Florida present a single-cell RNA amplification approach combining exponential and linear amplification steps using a limited number of PCR cycles and T7-driven in vitro transcription (IVT). Thier approach allows for unlimited ...
Read More »Consistent Errors in First Strand cDNA Due to Random Hexamer Mispriming
Priming of random hexamers in cDNA synthesis is known to show sequence bias, but in addition it has been suggested recently that mismatches in random hexamer priming could be a cause of mismatches between the original RNA fragment and observed ...
Read More »