Motivated by the lack of easily implementable and generally applicable strategies to increase and assess data accuracy, researchers from the University of Georgia devised a novel label-free approach, termed REQUIEM, to address challenges in relative quantitation. For comparing the relative ...
Read More »SeqTU – A Web Server for Identification of Bacterial Transcription Units
A transcription unit (TU) consists of K ≥ 1consecutive genes on the same strand of a bacterial genome that are transcribed into a single mRNA molecule under certain conditions. Their identification is an essential step in elucidation of transcriptional regulatory networks. A ...
Read More »timeSeq – detect biologically relevant DE genes and gene sets in time course RNA-Seq data
Accurate identification of differentially expressed (DE) genes in time course RNA-Seq data is crucial for understanding the dynamics of transcriptional regulatory network. However, most of the available methods treat gene expressions at different time points as replicates and test the ...
Read More »RARseq – an RNAseq-based genotype by sequencing (GBS) method
Genotyping by sequencing (GBS) methods include genome reduction by restriction digestion. However, the amount of DNA after reduction may still be large and yield too much sequence per sample. Many of the identified SNPs are located within non-genic regions, and ...
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