Tag Archives: University of Michigan

scPLS – Controlling for confounding effects in single cell RNA sequencing studies using both control and target genes

rna-seq

Single cell RNA sequencing (scRNAseq) technique is becoming increasingly popular for unbiased and high-resolutional transcriptome analysis of heterogeneous cell populations. Despite its many advantages, scRNAseq, like any other genomic sequencing technique, is susceptible to the influence of confounding effects. Controlling ...

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Capture RNA-seq for degraded and clinical RNA

rna-seq

RNA-seq by poly(A) selection is currently the most common protocol for whole transcriptome sequencing as it provides a broad, detailed, and accurate view of the RNA landscape. Unfortunately, the utility of poly(A) libraries is greatly limited when the input RNA ...

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RNA Sequencing and the Evolution of Plants

rna-seq

Many phylogenomic studies based on transcriptomes have been limited to “single-copy” genes due to methodological challenges in homology and orthology inferences. Only a relatively small number of studies have explored analyses beyond reconstructing species relationships. A multi-national team led by ...

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