RNA-Seq Blog Transcriptome Research & Industry News
Experimental procedures for preparing RNA-seq and single-cell (sc) RNA-seq libraries are based on assumptions regarding their underlying enzymatic reactions. Here, researchers at the University of Warwick show that the fairness of these assumptions varies within libraries: coverage by sequencing reads ...
Read More »Polymerase processivity is the mechanistic origin of coverage biases during scRNA-Seq library preparation
