High throughput sequencing technology enables the both the human genome and transcriptome to be screened at the single nucleotide resolution. Tools have been developed to infer single nucleotide variants (SNVs) from both DNA and RNA sequencing data. To evaluate how ...
Read More »On-line calculator to determine the optimal sample size for a RNA-seq study
As RNA-seq rapidly develops and costs continually decrease, the quantity and frequency of samples being sequenced will grow exponentially. With proteomic investigations becoming more multivariate and quantitative, determining a study’s optimal sample size is now a vital step in experimental ...
Read More »An RNA-Seq QC Overview
Quality control (QC) is a critical step in RNA sequencing (RNA-seq). Yet, it is often ignored or conducted on a limited basis. Here, Vanderbilt University researchers present a multi-perspective strategy for QC of RNA-seq experiments. The QC of RNA-seq can ...
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