SAN CARLOS, Calif. & PISCATAWAY, N.J.–(BUSINESS WIRE)–A multi-site study has clinically validated an end-to-end workflow for the collection, storage, transport and preparation of human whole blood samples for RNA-Sequencing (RNA-Seq). The study, initiated in January 2015, integrated products from PreAnalytiX ...
Read More »RNA Bioinformatics – New RNA-Seq Protocols from Methods in Molecular Biology
RNA Bioinformatics Edited by Ernesto Picardi ISBN: 978-1-4939-2290-1 (Print) 978-1-4939-2291-8 (Online) Analysis of High-Throughput RNA Sequencing Data Quality Control of RNA-Seq Experiments Xing Li, Asha Nair, Shengqin Wang, Liguo Wang Pages 137-146 Accurate Mapping of RNA-Seq Data Kin Fai Au ...
Read More »Kapa Biosystems Expands Partnership with Roche NimbleGen to Include RNA Sample Preparation for Targeted Next-Gen Sequencing
Customized version of KAPA Stranded RNA-Seq Kit enhances Roche NimbleGen SeqCap RNA product offering December 08, 2014 08:00 AM Eastern Standard Time WILMINGTON, Mass.–(BUSINESS WIRE)–Kapa Biosystems, Inc. today announced an expanded partnership with Roche NimbleGen to include new, optimized, RNA ...
Read More »Overview of an RNA Seq Workflow
This series is part of the Bioconductor Course held in Seattle from Oct 27 to Oct 28, 2014. In this video Martin Morgan, gives an overview of a typical RNA Seq workflow. Material for this video can be found at: ...
Read More »HTSeq – a Python framework to work with high-throughput sequencing data
A large choice of tools exists for many standard tasks in the analysis of high-throughput sequencing (HTS) data. However, once a project deviates from standard workflows, custom scripts are needed. Researchers at the European Molecular Biology Laboratory present HTSeq, a ...
Read More »SeqAssist – a novel toolkit for preliminary analysis of next-generation sequencing data
While next-generation sequencing (NGS) technologies are rapidly advancing, an area that lags behind is the development of efficient and user-friendly tools for preliminary analysis of massive NGS data. As an effort to fill this gap to keep up with the ...
Read More »New library sample preparation kits for Next Generation Sequencing announced by AMSBIO
AMSBIO announces RapidSeq – new convenient and cost-effective library sample preparation kits for Next Generation Sequencing (NGS) research and diagnostic work. The RapidSeq Small RNA Sample Prep Kit is optimised for preparation of NGS libraries for subsequent cluster generation, using ...
Read More »Online games and remote experiments could reduce scientific fraud, cherry-picking
Carnegie Mellon and Stanford researchers advocate massive online laboratories PITTSBURGH—One way to combat the rising level of errors and fraud in life sciences research is through massive online laboratories, which use videogames to engage large numbers of non-professional investigators and ...
Read More »RVboost: RNA-Seq variants prioritization using a boosting method
RNA-Seq has become the method of choice to quantify genes and exons, discover novel transcripts, and detect fusion genes. However, reliable variant identification from RNA-Seq data remains challenging due to the complexities of the transcriptome, the challenges of accurately mapping ...
Read More »Success in RNA-Seq – Sample Prep and Workflow considerations
by Natalie McDonald, PhD RNA in the cell is comprised of a variety of populations, including traditional poly-adenylated messenger RNA (polyA RNA), non-adenlyated RNA, which may be coding or regulatory, ribosomal RNA (rRNA) and small and micro RNA. The first ...
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