The RNA-seqlopedia – an overview of RNA-seq and of the choices necessary to carry out a successful RNA-seq experiment

RNA-seq produces millions of sequences from complex RNA samples. With this powerful approach, you can:

1. Measure gene expression.
2. Discover and annotate complete transcripts.
3. Characterize alternative splicing and polyadenylation.

The RNA-seqlopedia provides an overview of RNA-seq and of the choices necessary to carry out a successful RNA-seq experiment.

RNA-seqlopedia is written by the Cresko Lab of the University of Oregon and was funded by grant R24 RR032670 (NIH, National Center for Research Resources).

A typical RNA-seq experiment consists of the following steps:

Each of the five chapters below are dedicated to one of these steps.

1. Experimental Design
2. RNA Preparation
3. Library Preparation
4. 4Sequencing
5. Analysis
6. References