RNA sequencing (RNA-seq) has become the standard for expression profiling methods in cells, tissues and complete organisms. We have developed an elegant microfluidics-based chemistry and workflow—the Advanta™ RNA-Seq XT NGS Library Prep Kit. Leveraging the Juno™ instrument, our RNA-Seq workflow supports simultaneous processing of up to 48 samples with a one-click script. We have recently updated our kits to include unique dual index (UDI) adapters, ideal for use on Illumina® NovaSeq™ platforms, and we have simplified the workflow for additional savings of hands-on time and labor costs.
- Comparisons for TruSeq® Stranded mRNA LP, NEBNext® Ultra™ II Directional RNA LP and KAPA mRNA HyperPrep
- All workflows include quantification of 48 samples by qPCR and pooling to obtain the final library for sequencing
Advanta RNA-Seq XT NGS Library Prep Kits include reagents necessary to generate random-primed, stranded RNA-seq libraries from the polyadenylated RNA fraction present in as low as 10 ng of total RNA from eukaryotic organisms. In this webinar we will show performance data from several of our internal studies as well as from early-access customers. We will also describe studies in progress to utilize RNA-seq for analysis of the host immune response in COVID-19 subjects.
Date: September 17, 2020
Time: 12:00-13:00 PDT I 21:00-22:00 CET I 20:00-21:00 BST
Speaker: Steven Kain, PhD, MBA – Director of Product Management, FluidigmREGISTER