RNA-Seq Blog Transcriptome Research & Industry News
Discover More in Transcriptome Research with the Unprecedented Read Coverage, Sensitivity and Resolution of Targeted RNA Sequencing
Date: Wednesday, November 12, 2014
Time: 3pm EST (12pm PST)
Duration: 90 Minutes
Featured Speakers:
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While researchers are currently able to perform whole-transcriptome sequencing (RNA-Seq) to evaluate expression levels, variant splicing events, SNPs, and InDels, the standard RNA-Seq approach has significant drawbacks. A major challenge is that many rare events are not detected due to the depth of sequencing necessary to resolve them whereas the overwhelming number of sequencing reads derive from a small subset of highly abundant transcripts.
Dr. Mercer and Dr. Tan will present a novel targeted RNA sequencing method and elaborate on experiences with targeted RNA sequencing that overcomes many of the standard RNA-Seq limitations and achieves unprecedented read coverage, sensitivity and resolution.
Targeted RNA sequencing enables more sensitive gene discovery, more precise measurement of gene abundance, and more accurate isoform assembly. Data will be presented on targeted RNA sequencing to (i) annotate long noncoding RNAs, (ii) profile the expression and aberrant splicing of oncogenes in tumors, (iii) discover new genes in ‘empty’ disease-associated genome intervals and (iv) map transient RNA intermediates in the splicing pathway. For instance, an analysis of transcription from human chromosome 21 reveals a massive abundance and diversity of splicing, coding and noncoding RNAs, well beyond current annotations. Further comparison to the syntenic mouse transcriptome distinguishes the evolutionary forces shaping this transcriptional and regulatory complexity.
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