Monday – Sept 27th, 2021
11:00 AM – 12:00 PM Eastern Time
Since its development a decade ago, researchers have developed a plethora of RNA sequencing (RNA-seq)-based technologies for studying RNA biology and the transcriptome. However, each technology is designed to answer a particular research question, so researchers must make specific changes to their experimental setup and RNA library preparation to tailor RNA-seq to their needs. This Technique Talk will explore how to select the RNA sequencing platform best suited for a scientific question and how to adapt experimental design according to downstream applications.
- Available RNA sequencing technologies and their applications
- Optimizing library preparation depending on the sequencing platform
- How to obtain the proper sample quality required for each RNA sequencing technology
- The importance of replicate numbers to obtain reliable results