The authors describe a protocol for the Illumina Genome Analyzer II platform for mRNA-Seq sequencing for library preparation that avoids significant PCR amplification and requires only 10 nanograms of total RNA. While this protocol has been described previously and validated for single-end sequencing, where it was shown to produce directional libraries without introducing significant amplification bias, here they validate it further for use as a paired end protocol.
RNA modifications poster – Discover the chemical structure of different RNA modifications and their distribution and function in mRNA and tRNAs
July 17, 2018
December 7, 2017
August 11, 2017
July 26, 2017