Visualizing the transcriptome : A comparison of different RNA library preparation methods

RNA-Seq is a powerful tool used to obtain in-depth information on expression profiling, gene annotation, and transcript discovery. With the growing popularity of RNA sequencing, new library preparation techniques are becoming commercially available. These techniques are improvements on the classic poly-A selection and rRNA reduction methods, and in some cases sensitive enough to analyze the transcriptome of a single cell. However, limited information is available on comparative analysis of these methods.