Although RNA-seq is a powerful tool, it can present bioinformatic challenges. Whether you’re a seasoned researcher or a transcriptomics newbie, an in-depth look at the complexities of RNA-seq analysis can be useful for any investigation into gene expression profiling.
Federica Torri, a Genomic Scientist and Project Manager at Seven Bridges Genomics, provided an in-depth look at a range of RNA-seq issues in The Hows and Whys of RNA-Seq, including:
· RNA-seq quantitation
· Differential expression analysis
· Transcriptome assembly
· Challenges of genome-guided reconstruction
· Protocols and visualization tools
Feel free to review the slides and recording and get in touch if you have any questions or comments!