On 24 and 25 June, NeSI and Genomics Aotearoa will be delivering an online workshop on RNA Sequencing.

The workshop will be taught over two mornings from 9:00am – 1:00pm NZT on both days.

The focus of this workshop is to analyse RNA-seq data with the aim to identify differentially expressed genes. By the end of this workshop you will have a good understanding of how differentially expressed genes are identified and be able to choose the methods and tools that suit your experimental design.

On Day 1: we will cover quality control and read cleaning, alignment and quantification methods, and carry out an exploratory analysis of an example data set.

On Day 2:  we will work through two methods which take unique statistical approaches to identifying differentially expressed genes. We will then look at over-representation analysis, a popular method to derive biological meaning from a list of differentially expressed genes.

Who this workshop is for?

This is a beginner-friendly workshop which assumes you are familiar with the basics of R ( e.g., you can copy basic functions like head, tail, or colSums, you are aware of how R stores files as Objects) and bash (e.g., can change directory with cd command, make a new folder with mkdir command, view contents using less command). If you would like a refresher on R you can find one here. If you would like a refresher on bash you can find one here.

Setup

• This is a fully online, hands-on workshop.
• This workshop material will delivered on the NeSI High Performance Computing (HPC) platform – there is no need to install any software for this workshop. Instructions on how to access the platform will be sent out with the confirmation letter to registrants.
• Participants must have their own laptops and plan to participate actively. You will require a working web browser.
• If you have any questions about these workshops, including whether they are suitable for you, please contact training@nesi.org.nz